Anti-IRE1 (phospho S724) antibody ab48187 from Abcam

Product Specs
ItemAnti-IRE1 (phospho S724) antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab48187
Antibody TypePrimary
HostRabbit
Quantity100 UL
ClonalityPolyclonal
ApplicationsELISA, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry paraffin embedded sections, Western blot
IsotypeIgG
Conjugate/TagUnconjugated
Concentration1 mg/ml
ModificationsPhospho-S724
Gene NameERN1
ReactivityAfrican green monkey, Human, Mouse
TargetIRE1

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) STEADY-STATE REGULATION OF COPII-DEPENDENT SECRETORY CARGO SORTING BY INOSITOL TRISPHOSPHATE RECEPTORS, CALCIUM, AND PENTA EF HAND PROTEINSJournal of Biological ChemistryNovember 17, 2023Aaron Held, Jacob Lapka, John Sargeant, Jennet Hojanazarova, Alaa Shaheen, Samuel Galindo, Corina Madreiter-Sokolowski, Roland Malli, Wolfgang F. Graier, Jesse C. HayCell Signaling Technology (product 9721s). Rabbit polyclonal anti Phospho-IRE1 was purchased from Abcam (item ab48187). Rabbit polyclonal anti-ATF4 antibody was purchased from GeneTex (Ca2+; product GTX101943). Rabbit

(2) Acute Endoplasmic Reticulum Stress Suppresses Hepatic Gluconeogenesis by Stimulating MAPK Phosphatase 3 DegradationInternational Journal of Molecular SciencesOctober 25, 2023Xiaohua Huang, Heng Zhu, Wei Lu, Lei Cao, Zhengfeng Fang, Lianqiang Che, Yan Lin, Shengyu Xu, Yong Zhuo, Lun Hua, et al.were purchased from Santa Cruz Biotechnology (Santa Cruz, Dallas, TX, USA), anti-phospho-IRE1 (Ser724, ab48187) antibody was from Abcam (Cambridge, MA, USA), anti-sXBP1 (#83418), anti-phospho-PERK (Thr980, #3179s)

(3) Endoplasmic Reticulum Stress Promotes the Expression of TNF-α in THP-1 Cells by Mechanisms Involving ROS/CHOP/HIF-1α and MAPK/NF-κB PathwaysInternational Journal of Molecular SciencesOctober 14, 2023Nadeem Akhter, Ajit Wilson, Hossein Arefanian, Reeby Thomas, Shihab Kochumon, Fatema Al-Rashed, Mohamed Abu-Farha, Ashraf Al-Madhoun, Fahd Al-Mulla, Rasheed Ahmad, et al.(Cat. #9102), phospho-NF-κB (Cat. #3031), and total- NF-κB (Cat. #3034s). Phospho (S724)-IRE1α (Cat. #ab48187) was purchased from Abcam (Waltham, MA, USA). Blots were washed 3 times with TBS wash buffer and incubated

(4) Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1α/ASK1/JNK signaling pathway in mice with traumatic brain injuryJournal of neuroinflammationSeptember 30, 2023Shi G, Liu L, Cao Y, Ma G, Zhu Y, Xu J, Zhang X, Li T, Mi L, Jia H, et al.(1:1000, ab216327, Abcam, UK), goat anti-Iba-1 (1:2000, ab5076, Abcam, UK), rabbit anti-pIRE1α (1:1000, ab48187, Abcam, UK), rabbit anti-IRE1α (1:1000, ab37073, Abcam, UK), rabbit anti-pASK1(1:1000, ab278547, Abcam,

(5) Non-small cell lung cancers (NSCLCs) oncolysis using coxsackievirus B5 and synergistic DNA-damage response inhibitorsSignal Transduction and Targeted TherapySeptember 25, 2023Bopei Cui, Lifang Song, Qian Wang, Kelei Li, Qian He, Xing Wu, Fan Gao, Mingchen Liu, Chaoqiang An, Qiushuang Gao, et al.p-PERK (1: 1000, 3179, Cell Signaling Technology), IRE1 (1: 2000, ab96481, Abcam), p-IRE1 (1: 2000, ab48187, Abcam), ATF6 (1: 2000, ab227830, Abcam), eIF2α (1: 2000, 5324, Cell Signaling Technology), p-eIF2α

(6) Combination of RUNX1 inhibitor and gemcitabine mitigates chemo-resistance in pancreatic ductal adenocarcinoma by modulating BiP/PERK/eIF2α-axis-mediated endoplasmic reticulum stressJournal of experimental & clinical cancer research : CRSeptember 11, 2023She C, Wu C, Guo W, Xie Y, Li S, Liu W, Xu C, Li H, Cao P, Yang Y, et al.for Western blot were as follow: RUNX1 (ab23980, Abcam), RUNX1 (ab189172, Abcam), BiP (ab21685, Abcam), p-IRE1α (ab48187,S724, Abcam), ATF6 (ab227830, Abcam), XBP-1(sc-8015, Santa cruz) and the antibodies from Cell Signaling

(7) Hepatic levels of S-adenosylmethionine regulate the adaptive response to fastingCell metabolismAugust 8, 2023Capelo-Diz A, Lachiondo-Ortega S, Fernández-Ramos D, Cañas-Martín J, Goikoetxea-Usandizaga N, Serrano-Maciá M, González-Rellan MJ, Mosca L, Blazquez-Vicens J, Tinahones-Ruano A, et al.RRID:AB_2107448 GRP75,Cell Signaling Technology,Cat#3593; RRID:AB_2120328 Phospho-Ire1α,Abcam,Cat#Ab48187; RRID:AB_873899 Ire1α,Santa Cruz,Cat#SC-390960; RRID:AB_2927490 IP3R,Abcam,Cat#ab5804; RRID:AB_305124 MATα1,In-house,N/A MATα2,Abcam,Cat#ab26174;

(8) Unfolded protein response factor ATF6 augments T helper cell responses and promotes mixed granulocytic airway inflammationMucosal immunologyAugust 1, 2023Wu D, Zhang X, Zimmerly KM, Wang R, Wang C, Hunter R, Wu X, Campen M, Liu M, Yang XO.anti-ATF6 (A12570, ABclonal), anti-Xbp1s (ab220783, Abcam), anti-IRE1 (NB100–2324, Novus), anti-p-IRE1 (ab48187, Abcam), anti-p-PERK (bs-3330R-TR, Bioss), anti-PERK (bs-2469R-TR, Bioss), anti-GATA3 (16E10A23, BioLegend),

(9) Targeting extracellular CIRP with an X-aptamer shows therapeutic potential in acute pancreatitisiScienceJuly 21, 2023Liu W, Bi J, Ren Y, Chen H, Zhang J, Wang T, Wang M, Zhang L, Zhao J, Wu Z, et al.RRID:AB_2651017 Anti-Mfn-2,Abcam,Cat ab124773; RRID:AB_10999860 Anti-IRE1α,Abcam,Cat ab235171 Anti-p-IRE1α,Abcam,Cat ab48187; RRID:AB_873899 Anti-CIRP,Abcam,Cat ab246510; RRID:AB_2923244 Anti-TLR4,Proteintech,Cat 66350-1-Ig;

(10) Unconventional Activation of IRE1 Enhances TH17 Responses and Promotes Neutrophilic Airway InflammationbioRxiv : the preprint server for biologyJuly 8, 2023Wu D, Zhang X, Zimmerly KM, Wang R, Livingston A, Iwawaki T, Kumar A, Wu X, Mandell MA, Liu M, et al.and IL-23R (12B2B64) were purchased from eBioscience. Anti-XBP1 (ab220783) and anti-p-IRE1 (S724) (ab48187) were from Abcam, and p-JAK2 (Y1007, Y1008) (44–426G) from Invitrogen. Phospho-protein stain was performed

(11) A novel IRE1 kinase inhibitor for adjuvant glioblastoma treatmentiScienceMay 19, 2023Diana Pelizzari-Raymundo, Dimitrios Doultsinos, Raphael Pineau, Chloé Sauzay, Thodoris Koutsandreas, Timothy Langlais, Antonio Carlesso, Elena Gkotsi, Luc Negroni, Tony Avril, et al.polyclonal; SantaCruz Biotechnologies, H-190) and pS724-IRE1 antibody (Anti-human; rabbit polyclonal; Abcam, ab48187), respectively. The phosphorylated form of eIF2α was stained with anti-phospho-eIF2α (Ser51) Antibody

(12) Indicators of increased ER stress and UPR in aged D2-mdx and human dystrophic skeletal musclesFrontiers in PhysiologyApril 25, 2023Swathy Krishna, Hannah R. Spaulding, James E. Koltes, John C. Quindry, Rudy J. Valentine, Joshua T. Selsbysignaling,Rabbit,1:1000 TBST,1:2000 TBST peIF2α S51,"3,398",Cell signaling,Rabbit,1:1000 TBST,1:2000 TBST pIRE1α S724,Ab48187,Abcam,Rabbit,"1:1,000 2.5% milk",1:1000 TBST PKR,SC-6282,Santa cruz biotechnology,Rabbit,1:1000 TBST,1:2000

(13) PROTAC-Mediated Selective Degradation of Cytosolic Soluble Epoxide Hydrolase Enhances ER Stress ReductionACS chemical biologyApril 21, 2023Wang Y, Morisseau C, Takamura A, Wan D, Li D, Sidoli S, Yang J, Wolan DW, Hammock BD, Kitamura S.following antibodies were used, including: 1) a previously generated sEH antibody 51; 2) phospha-IRE1α (#ab48187, Abcam, Waltham, MA); 3) IRE1α (#3294, Cell Signaling, Danvers, MA); 4) XBP-1s (#12782, Cell Signaling,

(14) Modulation of the endoplasmic reticulum stress and unfolded protein response mitigates the behavioral effects of early-life stressPharmacological reports : PRApril 1, 2023Solarz-Andrzejewska A, Majcher-Maślanka I, Kryst J, Chocyk A.# GRP78/HSPA5,Rabbit,1:1000,Cell Signaling Tech,3183 IRE1α,Rabbit,1:1000,Abcam,ab37073 p-IRE1α (S724),Rabbit,1:1000,Abcam,ab48187 PERK,Rabbit,1:1000,Cell Signaling Tech,3192 p-PERK (Thr980),Rabbit,1:1000,Cell Signaling Tech,3179 ATF6,Rabbit,1:1000,Proteintech,24169–1-AP eIF2α,Rabbit,1:2000,Cell Signaling

(15) Deubiquitylase OTUD3 Mediates Endoplasmic Reticulum Stress through Regulating Fortilin Stability to Restrain Dopaminergic Neurons ApoptosisAntioxidants (Basel)March 26, 2023Ling Chen, Xuejie Huan, Fengju Jia, Zhen Zhang, Mingxia Bi, Lin Fu, Xixun Du, Xi Chen, Chunling Yan, Qian Jiao, et al.anti-PERK (1:000, 3192, Cell Signaling Technologies, Danvers, MA, USA); Rabbit anti-phospho-IRE1α (1:000, ab48187, Abcam, UK), Rabbit anti-IRE1α (1:000, #3294, Cell Signaling Technologies, Danvers, MA, USA); Rabbit

(16) Deficiency of Perry syndrome-associated p150(Glued) in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalitiesNPJ Parkinson's diseaseMarch 7, 2023Yu J, Yang X, Zheng J, Sgobio C, Sun L, Cai H.#ab32157, 1:500], and phosphorylated inositol-requiring enzyme 1α (Ser724) [p-IRE1α (Ser724), Abcam, #ab48187, 1:500] as suggested by manufacturers. Alexa Fluor 488-, 546-, or 647-conjugated secondary antibody

(17) Ethanol and its Nonoxidative Metabolites Promote Acute Liver Injury by Inducing ER Stress, Adipocyte Death, and LipolysisCellular and Molecular Gastroenterology and HepatologyJanuary 1, 2023Seol Hee Park, Wonhyo Seo, Ming-Jiang Xu, Bryan Mackowiak, Yuhong Lin, Yong He, Yaojie Fu, Seonghwan Hwang, Seung-Jin Kim, Yukun Guan, et al.PERK (C33E10) (#3192; CST), p-eIF2α (Ser51) (#3398; CST), eIF2α (#5324; CST), p-IRE1α (phospho-S724) (ab48187; Abcam), IRE1α (14C10) (#3294; CST), X-box binding protein 1 (E9V3E) (#40435; CST), CHOP (#5554s; CST),

(18) NADPH oxidase and endoplasmic reticulum stress is associated with neuronal degeneration in orbitofrontal cortex of individuals with alcohol use disorderAddiction biologyJanuary 1, 2023Qin L, Vetreno RP, Crews FT."Novus Biologicals, #NBP1‐80930",IHC (Novus Biologicals) pIRE1 (phospho S724),Rabbit,Polyclonal,1:100,"Abcam Inc., #ab48187",WB (Abcam) ATF6,Rabbit,Polyclonal,1:150,"Abcam Inc., #ab203119",WB (Abcam) CHOP,Rabbit,Polyclonal,1:00,"Abcam

(19) Alterations in UPR Signaling by Methylmercury Trigger Neuronal Cell Death in the Mouse BrainInternational Journal of Molecular SciencesDecember 6, 2022Ryosuke Nomura, Nobumasa Takasugi, Hideki Hiraoka, Yuta Iijima, Takao Iwawaki, Yoshito Kumagai, Masatake Fujimura, Takashi Ueharaenzyme 1 (HRD1) (1:500; #sc-130889, Santa Cruz Biotechnology, Dallas, TX, USA), anti-p-IRE1α (1:1200; #ab48187, Abcam, Cambridge, UK) and anti-p-PERK (1:2000; #sc-32577, Santa Cruz Biotechnology, Dallas, TX, USA)

(20) Resistance Training Modulates Reticulum Endoplasmic Stress, Independent of Oxidative and Inflammatory Responses, in Elderly PeopleAntioxidants (Basel, Switzerland)November 14, 2022Estébanez B, Visavadiya NP, Vargas JE, Rivera-Viloria M, Khamoui AV, de Paz JA, Huang CJ.(Ref. sc-293072) (Santa Cruz Biotechnology, Dallas, TX, USA); IRE1 (Ref. ab37073), and pIRE1 (Ref. ab48187) (Abcam); TRAF6 (Ref. 8028) and β-tubulin (Ref. 3700) (Cell Signaling Technology, Danvers, MA, USA);

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8/25/2016Phosphorylated IRE1alpha Claire StewartIRE1alpha is a key protein that is phosphorylated and activated during ER stress, initiating the unfolded protein response. Determining the activation state of IRE1alpha due to a variety of stress ... Read Review

Anti-IRE1 (phospho S724) antibody from Abcam

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Anti-IRE1 (phospho S724) antibody from Abcam

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